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Several studies have reported the presence of an inhibitory substance in the preovulatory follicular fluid that is thought to delay egg activation by a direct action on the follicular cells. The effect of this substance has been termed "follicular inhibition", and its presence and molecular characteristics have been well established. However, the lack of specific antigen has hampered the development of an effective immunoassay, and has thereby prevented the detection of follicular inhibition in plasma from ovulating birds. We therefore assessed the importance of follicular inhibition in the control of egg activation by monitoring the effects of a "follicular inhibition-like" substance on chicken eggs using adenosine triphosphate (ATP) as a direct stimulus to egg activation 0b46394aab